Dr. Refaat Hamed,Assistant Professor
Information about Dr. Refaat Hamed at the University of Bradford.
- School of Chemistry & Biosciences
(Faculty of Life Sciences) - Email:
- r.farghal@bradford.ac.uk
- Telephone:
- +44 1274 232371
Biography
I have always been fascinated by the way nature morphs simple building blocks to create more complex natural cures. This fascination shaped my career, which started by the isolation, structure elucidation and biological evaluation of various classes of phytochemicals, with the focus being iridoid glycosides (MSc studies). I then moved on to pursue my D.Phil. studies at University of Oxford, where I learned the modern aspects of (b-lactam antibiotics) biosynthesis, molecular biology and enzymology (structural and mechanistic). During my D.Phil. and postdoctoral studies time, I have developed myself into a rational-design protein engineer. Challenging chemical transformations and forcing enzymes, via engineering, to catalyse these reactions, with exquisite regio- and stereoselectivity, for biocatalytic purposes, is one of the main themes of my research. Extending my research from engineering enzymes to redesign of biosynthetic pathways to produce unnatural products of pharmaceutical interest is another objective for my research. As a teacher, I do strive to be well-informed, interactive and inspiring. One of my primary goals of teaching is to prepare both undergraduate and graduate students for a successful career.Research
Natural products discovery, Biosynthesis of natural products, Biocatalysis, Enzymology, Protein Engineering and Synthetic BiologyResearch projects
Role | Date | Title/description | Funder | Award |
---|---|---|---|---|
2019-02-01T00:00:00 | http://gtr.rcuk.ac.uk/project/19D7A33F-E0B6-432C-B924-70F8EA55B953 |
Teaching
Details on teaching interests, highlights and modules are available for Dr. Refaat Hamed as follows:
Teaching interests
Organic Chemistry, Medicinal Chemistry, BiochemistryTeaching highlights
NMR SpectroscopyCarbonyl Chemistry
Stereoselective Synthesis
Retrosynthesis
Professional activities
Information about education, employment and areas of particular interest for Dr. Refaat Hamed is as follows:
Employment
- Assiut University, Egypt - Associate Professor (On leave) in the year 2016 (specified as 01/07/2016)
- Assiut University, Egypt - Assistant Professor in the year 2011 (specified as 01/06/2011)
- Assiut University, Egypt - Assistant lecturer in the year 2004 (specified as 01/04/2004)
- Assiut University, Egypt - Demonstrator in the year 1999 (specified as 01/12/1999)
- University of Bradford - Lecturer of Chemical Biology in the year 2018 (specified as 27/02/2018)
- University of St Andrews - Research Fellow in the year 2016 (specified as 16/01/2016)
- University of Illinois Urbana Champaign - Research Fellow in the year 2013 (specified as 30/05/2013)
- University of Oxford - Research Fellow in the year 2010 (specified as 10/03/2010)
Education
- University of Oxford - DPhil Organic Chemistry
- Assiut University - MSc in Natural Products Chemistry
- Assiut University - BSc in Pharmaceutical Sciences (with highest honors)
Publications
There are 24 publications involving or that are attributed to Dr. Refaat Hamed.
Peer Reviewed Journal
Title | Year | Publication name | Journal | Volume | Pages | Authors | Editors | ISSN | Publisher | DOI | Location |
---|---|---|---|---|---|---|---|---|---|---|---|
Self-hydroxylation of the splicing factor lysyl hydroxylase, JMJD6 | 2012 | MedChemComm | Mantri, M.;Webby, C.J.;Loik, N.D.;Hamed, R.B.;Nielsen, M.L.;McDonough, M.A.;McCullagh, J.S.O.;Böttger, A.;Schofield, C.J.;Wolf, A.; | 10.1039/c1md00225b | |||||||
Oxygenase-catalyzed ribosome hydroxylation occurs in prokaryotes and humans | 2012 | Nature Chemical Biology | Ge, W.;Wolf, A.;Feng, T.;Ho, C.-H.;Sekirnik, R.;Zayer, A.;Granatino, N.;Cockman, M.E.;Loenarz, C.;Loik, N.D.;Hardy, A.P.;Claridge, T.D.W.;Hamed, R.B.;Chowdhury, R.;Gong, L.;Robinson, C.V.;Trudgian, D.C.;Jiang, M.;MacKeen, M.M.;McCullagh, J.S.;Gordiyenko, Y.;Thalhammer, A.;Yamamoto, A.;Yang, M.;Liu-Yi, P.;Zhang, Z.;Schmidt-Zachmann, M.;Kessler, B.M.;Ratcliffe, P.J.;Preston, G.M.;Coleman, M.L.;Schofield, C.J.; | 10.1038/nchembio.1093 | |||||||
Factor-inhibiting hypoxia-inducible factor (FIH) catalyses the post-translational hydroxylation of histidinyl residues within ankyrin repeat domains | 2011 | FEBS Journal | Yang, M.;Chowdhury, R.;Ge, W.;Hamed, R.B.;McDonough, M.A.;Claridge, T.D.W.;Kessler, B.M.;Cockman, M.E.;Ratcliffe, P.J.;Schofield, C.J.; | 10.1111/j.1742-4658.2011.08022.x | |||||||
Use of Methylmalonyl-CoA Epimerase in Enhancing Crotonase Stereoselectivity | 2016 | ChemBioChem | 17 | Hamed, Refaat B.; Gomez-Castellanos, J.R.; Sean Froese, D.; Krysztofinska, E.; Yue, W.W.; Schofield, C.J. | |||||||
Stereoselective preparation of lipidated carboxymethyl-proline/pipecolic acid derivatives via coupling of engineered crotonases with an alkylmalonyl-CoA synthetase | 2013 | Organic and Biomolecular Chemistry | Hamed, R.B.;Henry, L.;Gomez-Castellanos, J.R.;Asghar, A.;Brem, J.;Claridge, T.D.W.;Schofield, C.J.; | 10.1039/c3ob41525b | |||||||
Crotonase catalysis enables flexible production of functionalized prolines and carbapenams | 2012 | Journal of the American Chemical Society | Hamed, R.B.;Henry, L.;Gomez-Castellanos, J.R.;MecinoviÄ, J.;Ducho, C.;Sorensen, J.L.;Claridge, T.D.W.;Schofield, C.J.; | 10.1021/ja208318d | |||||||
The enzymes of β-lactam biosynthesis | 2013 | Natural Product Reports | Hamed, R.B.;Gomez-Castellanos, J.R.;Henry, L.;Ducho, C.;McDonough, M.A.;Schofield, C.J.; | 10.1039/c2np20065a | |||||||
Living GenoChemetics by hyphenating synthetic biology and synthetic chemistry in vivo | 2017 | Nature Communications | 8 | Sharma, S.V.; Tong, X.; Pubill-Ulldemolins, C.; Cartmell, C.; Bogosyan, E.J.A.; Rackham, E.J.; Marelli, E.; Hamed, Refaat B.; Goss, R.J.M. | |||||||
Mechanisms and structures of crotonase superfamily enzymes - How nature controls enolate and oxyanion reactivity | 2008 | Cellular and Molecular Life Sciences | Hamed, R.B.;Batchelar, E.T.;Clifton, I.J.;Schofield, C.J.; | 10.1007/s00018-008-8082-6 | |||||||
The 2-Oxoglutarate-Dependent Oxygenase JMJD6 Catalyses Oxidation of Lysine Residues to give 5S-Hydroxylysine Residues | 2011 | ChemBioChem | Mantri, M.;Loik, N.D.;Hamed, R.B.;Claridge, T.D.W.;McCullagh, J.S.O.;Schofield, C.J.; | 10.1002/cbic.201000641 | |||||||
Synthesis of regio- and stereoselectively deuterium-labelled derivatives of l-glutamate semialdehyde for studies on carbapenem biosynthesis | 2009 | Organic and Biomolecular Chemistry | Ducho, C.;Hamed, R.B.;Batchelar, E.T.;Sorensen, J.L.;Odell, B.;Schofield, C.J.; | 10.1039/b903312b | |||||||
Evidence for the slow reaction of hypoxia-inducible factor prolyl hydroxylase 2 with oxygen | 2010 | FEBS Journal | Flashman, E.;Hoffart, L.M.;Hamed, R.B.;Bollinger Jr., J.M.;Krebs, C.;Schofield, C.J.; | 10.1111/j.1742-4658.2010.07804.x | |||||||
Stereoselective C-C bond formation catalysed by engineered carboxymethylproline synthases | 2011 | Nature Chemistry | Hamed, R.B.;Gomez-Castellanos, J.R.;Thalhammer, A.;Harding, D.;Ducho, C.;Claridge, T.D.W.;Schofield, C.J.; | 10.1038/nchem.1011 | |||||||
Photoactivable peptides for identifying enzyme-substrate and protein-protein interactions | 2011 | Chemical Communications | Rotili, D.;Altun, M.;Hamed, R.B.;Loenarz, C.;Thalhammer, A.;Hopkinson, R.J.;Tian, Y.-M.;Ratcliffe, P.J.;Mai, A.;Kessler, B.M.;Schofield, C.J.; | 10.1039/c0cc04457a | |||||||
Carboxymethylproline synthase catalysed syntheses of functionalised N-heterocycles | 2010 | Chemical Communications | Hamed, R.B.;MecinoviÄ, J.;Ducho, C.;Claridge, T.D.W.;Schofield, C.J.; | 10.1039/b924519g | |||||||
Monitoring the activity of 2-oxoglutarate dependent histone demethylases by NMR spectroscopy: Direct observation of formaldehyde | 2010 | ChemBioChem | Hopkinson, R.J.;Hamed, R.B.;Rose, N.R.;Claridge, T.D.W.;Schofield, C.J.; | 10.1002/cbic.200900713 | |||||||
Evidence that thienamycin biosynthesis proceeds via C-5 epimerization: ThnE catalyzes the formation of (2S,5S)-trans-carboxymethylproline | 2009 | ChemBioChem | Hamed, R.B.;Batchelar, E.T.;MecinoviÄ, J.;Claridge, T.D.W.;Schofield, C.J.; | 10.1002/cbic.200800652 | |||||||
Iron-mediated cleavage of C-C bonds in vicinal tricarbonyl compounds in water | 2009 | Angewandte Chemie - International Edition | MecinoviÄ, J.;Hamed, R.B.;Schofield, C.J.; | 10.1002/anie.200806296 | |||||||
Thioester hydrolysis and C-C bond formation by carboxymethylproline synthase from the crotonase superfamily | 2008 | Angewandte Chemie - International Edition | Batchelar, E.T.;Hamed, R.B.;Ducho, C.;Claridge, T.D.W.;Edelmann, M.J.;Kessler, B.;Schofield, C.J.; | 10.1002/anie.200803906 | |||||||
Biocatalytic production of bicyclic β-lactams with three contiguous chiral centres using engineered crotonases | 2019 | Communications Chemistry | 2 | Hamed, Refaat B.; Gomez-Castellanos, J.R.; Warhaut, H.L.; Claridge, T.D.W.; Schofield, C.J. | |||||||
Crotonases: Nature’s exceedingly convertible catalysts | 2017 | ACS Catalysis | 7 | Lohans, C.T.; Wang, D.Y.; Wang, J.; Hamed, Refaat B.; Schofield, C.J. | |||||||
Stereoselective production of dimethyl-substituted carbapenams via engineered carbapenem biosynthesis enzymes | 2017 | ACS Catalysis | 7 | Hamed, Refaat B.; Henry, L.; Claridge, T.D.W.; Schofield, C. | |||||||
A natural solution to photoprotection and isolation of the potent polyene antibiotic, marinomycin A | 2019 | Chemical Science | 10 | Christopher S. Bailey, Joseph S. Zarins-Tutt, Matthias Agbo, Hong Gao, Alberto Diego-Taboada, Maoluo Gan, Refaat B. Hamed, Emily R. Abraham, Grahame Mackenzie, P. Andrew Evans, Rebecca J. M. Goss; | 10.1039/C9SC01375J | ||||||
Studies on the selectivity of proline hydroxylases reveal new substrates including bicycles | 2019 | Bioorganic Chemistry | 94 | Tristan J. Smart, Refaat B. Hamed, Timothy D.W. Claridge, Christopher J. Schofield | 0045-2068 | 10.1016/j.bioorg.2019.103386 |